Gene Knockouts - Gene/trait Relationships

Reverse genetics - starts with a gene and identifies its function

Transposon used for insertional mutagenesis in transgenic plants

Maintain large population of "knock out" transgenic plants

Screen population with PCR primers that amplify gene of interest

Southern blot hybridization to identify plants having insertion

Analysis of plants with transposon insertion in gene of interest causing morphological, cellular, biochemical or molecular alterations

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