Tomato Gene Pool – via tissue culture methods

(Diagram showing that L. esculentum is sexually compatible with L. chmielewskii, L. hirsutum,
L. parviflorum, L. pennellii, L. cheesmanii, and L. pimpinellifolium. Hybrids produced via embryo culture expand the gene pool to include S. lycopersicoides, S. sitiens, L. chilense, and L. peruvianum. Hybrids produced via somatic hybridization expand the gene pool to include S. ochranthum and S. juglandifolium.)

image modified from Roger Chetelat, UC Davis

immature tomato seed used for tissue culture

shoot regeneration from tomato embryo callus

callus formation on immature tomato seed

photos by Bruce Thomas, UC Davis

undeveloped seed, embryo callus, regeneration of hybrid plants

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