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99 red books
After many backcrosses the tomato had
the Mi gene with over 99% tomato genes,
but still had hundreds of wild tomato
genes in its genome
Backcrosses to L. esculentum (bookstack)
Long Description: The cultivated tomato L. esculentum makes large red fruits and carries the mi allele for susceptibility to nematode infection. The wild tomato L. peruvianum makes small green fruits and carries the Mi allele for resistance to nematodes.
L. esculentum
(mi, mi)
L. peruvianum
(Mi, Mi)
New Tomato Line
with Mi gene
Long Description: The F1 hybrid bookstack is backcrossed repeatedly to the L. esculentum parent (represented as a red bookstack). Each generation is selected for plants that contain the Mi gene from the L. peruvianum parent. After many backcrosses the progeny is represented as a red bookstack with just one white book representing the DNA from the L. peruvianum parent which contains the desired Mi allele.
image modified from Peggy Lemaux http://ucbiotech.org/
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