Current Methods in Plant Biotechnology

Bruce R. Thomas

Index of Slides

  1. Current Methods in Plant Biotechnology
  2. Introduction
  3. Domestication of Corn
  4. Radiation used to increase genetic variability of crops
  5. Tomato Gene Pool – via traditional breeding methods
  6. Tissue Culture Methods
  7. Tomato Gene Pool – via tissue culture methods
  8. Genomics Methods
  9. Structural Genomics - Structure of entire genomes
  10. Structural Genomics Projects for Plants
  11. RFLP Marker Linked to Gene for Sucrose Accumulation in Tomato Fruits
  12. Marker-Assisted Breeding - Tomato Mi gene
  13. DNA sequence information - tomato genome (bookstack)
  14. Production of F1 hybrid (bookstack)
  15. Backcrosses to L. esculentum (bookstack)
  16. Mapping of the tomato Mi gene
  17. Functional Genomics
  18. Synteny aids in gene identification
  19. Gene Knockouts - Gene/trait Relationships
  20. DNA Microarrays - analysis of gene expression
  21. DNA Microarrays - Analysis of Gene Expression Throughout the Genome
  22. Genomics Will Accelerate Crop Improvement
  23. Transgenic Plant Methods
  24. Gene Transfer by Transgenic Plant Methods (bookstack)
  25. Transgenic plant methods expand the gene pool
  26. 1. Delivery of gene clone into target cells
  27. Transgenic plants produced via Agrobacterium
  28. Tomato transformation - germinate seeds
  29. Cut explants, expose to Agrobacterium
  30. Tomato Plant Regeneration
  31. Transfer Regenerated Plants To Soil
  32. Gene Transformation via Biolistic Method
  33. 2. Selection of transgenic cells
  34. 3 regeneration of fertile transgenic plants
  35. 4. Expression of gene clone in transgenic plants
  36. Promoters for expression of nuclear transgenes
  37. Summary

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