4. Expression of gene clone in transgenic plants

Transgenic Plant Methods

• Regulation of gene expression

promoters control initiation of transcription

control of mRNA turnover

control of protein turnover

• Control of protein localization

signal peptide for endoplasmic reticulum (N-terminal)

vacuole localization (C-terminal)

nuclear localization signal (internal, bipartite)

plastid transit peptide (N-terminal)

mitochondrial transit peptide (N-terminal)

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