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Transgenic plants produced via Agrobacterium
1. gene cloning in E. coli
Diagram of gene cloning in E. coli
Long Description: A restriction fragment of foreign DNA containing a gene of interest is inserted into a plasmid DNA and sealed with ligase. This construct is transformed into E. coli and grown up to produce many colonies of E. coli cells. Various methods (not shown) are used to determine which colony contains a plasmid with the desired gene insert. This colony is then grown into a large culture and used for purification of the desired plasmid DNA.
2. gene clone transferred into Agrobacterium
3. Agrobacterium transfers gene into plant cells
cartoon of Agrobacterium cell injecting DNA into a plant cell
Images compliments of UCD Biotechnology Program.
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