Transgenic plants produced via Agrobacterium

Slide 27 of 37

Images compliments of UCD Biotechnology Program.

cartoon of Agrobacterium cell injecting DNA into a plant cell

Long Description: A restriction fragment of foreign DNA containing a gene of interest is inserted into a plasmid DNA and sealed with ligase. This construct is transformed into E. coli and grown up to produce many colonies of E. coli cells. Various methods (not shown) are used to determine which colony contains a plasmid with the desired gene insert. This colony is then grown into a large culture and used for purification of the desired plasmid DNA.

1. gene cloning in E. coli

2. gene clone transferred into

Agrobacterium

3. Agrobacterium transfers

gene into plant cells

Slide Links:

Text Only Version Text Mostly Version Graphic Version