Crop Biotechnology Methods

Bruce R Thomas

Index of Slides

  1. Crop Biotechnology Methods
  2. Objectives
  3. Agricultural Technology
  4. All of Agriculture is Technology
  5. All crops have been genetically modified during domestication
  6. Wheat - a new species produced via breeding technology
  7. Seedless fruits: genetic modification produced via breeding technology
  8. Gene pool reduction during crop domestication
  9. Plant Breeding Technology
  10. Gene pools of tomato and wild related species
  11. Tomato Gene Pool increased via hybridization methods
  12. Plant Breeding Technology: 1) uses the genetic variability in the gene pool 2) for selection of superior crop plants 3) that have many significant genetic modifications
  13. Bio Technology Methods
  14. Crop Development via Breeding and Biotechnology
  15. Timeline for production of a new crop variety via breeding and/or biotechnology approaches
  16. Tissue Culture Methods
  17. Tissue grafting in tree and vine crops
  18. Tissue Culture Methods
  19. Tissue Culture Methods
  20. Micropropagation via tissue culture
  21. Triticale - breeders produce a new species of crop plant
  22. Tomato Gene Pool - expanded via tissue culture methods
  23. Embryo culture used to transfer Mi gene into tomato
  24. Recombinant DNA Methods
  25. DNA structure
  26. DNA is packaged in the cell nucleus as chromosomes
  27. DNA sequences encode protein sequences
  28. Recombinant DNA and gene cloning in E. coli
  29. Gene cloning methods: enable the study of DNA structure & function
  30. Genomics Methods
  31. Genomics and Proteomics
  32. DNA Sequencing of Entire Plant Genomes
  33. DNA Microarrays - the "Gene Chip" Analysis of Gene Expression Throughout the Genome
  34. Genomics Methods Are Accelerating Crop Improvement
  35. Use of genomics data for "Marker-Assisted Breeding"
  36. Nematode-resistance gene Mi linked to the Aps marker
  37. Mi gene transfer into tomato is a difficult problem
  38. DNA sequence information - tomato genome (bookstack)
  39. Production of F1 hybrid (bookstack)
  40. Backcrosses to L. esculentum (bookstack)
  41. NO TITLE
  42. Transgenic Plant Methods
  43. Transgenic Plants - Overview
  44. Transgenic Plants - Strategy
  45. The size of the gene pool limits the traits that can be developed in crop plants via traditional breeding and tissue culture methods
  46. Transgenic Plant Goals - expand the gene pool
  47. Transgenic Plant Goals - transfer trait into elite cultivar (bookstack)
  48. Transgenic Plant Methods
  49. 1 Gene Transfer into Plant Cells
  50. 2 Selection of Transgenic Cells and 3 Regeneration of Transgenic Plants
  51. 4 Expression of Gene Clone in Transgenic Plants
  52. Summary
  53. Plant evolution, breeding & biotech
  54. If you would like more information

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